AR-News: Early Primate Pregnancy Experiment funded by NIH

סמדר rumsiki at
Sat Sep 13 08:27:43 EDT 2003

From: primfocus at

The Journal of Clinical Endocrinology & Metabolism
Vol. 88, No. 9 4316-4323
Copyright © 2003 by The Endocrine Society 

Functional Differentiation of the Placental
Syncytiotrophoblast: Effect of Estrogen on Chorionic
Somatomammotropin Expression during Early Primate

Biljana Musicki, Gerald J. Pepe and Eugene D. Albrecht

Departments of Obstetrics, Gynecology, and
Reproductive Sciences and Physiology (B.M., E.D.A.),
Center for Studies in Reproduction, The University of
Maryland School of Medicine, Baltimore, Maryland
21201; and Department of Physiological Sciences
(G.J.P.), Eastern Virginia Medical School, Norfolk,
Virginia 23501 

Address all correspondence and requests for reprints
to: Eugene D. Albrecht, Ph.D., Department of
Obstetrics, Gynecology and Reproductive Sciences,
University of Maryland School of Medicine, Bressler
Research Laboratories 11-019, 655 West Baltimore
Street, Baltimore, Maryland 21201. E-mail:
ealbrech at

Estrogen stimulates morphological and functional (i.e.
steroidogenesis) differentiation of the primate
placental trophoblast, and with advancing gestation
there is an increase in estrogen and placental
chorionic somatomammotropin (CS) mRNA and protein
levels. To examine whether CS formation is regulated
by estrogen, placental villous trophoblast CS was
determined in baboons in which estradiol levels in
uterine vein were increased 2- to 3-fold (P < 0.01) on
d 60 of pregnancy (term = 184 d) by administration of
aromatizable androstenedione on d 30-59 or estradiol
benzoate on d 45-59 of gestation. Androstenedione and
estradiol treatment resulted in a 75% decrease (P <
0.01) in placental whole villous CS-3 mRNA and CS
protein levels, determined by Northern and Western
blot analysis, on d 60, and a corresponding decrease
in syncytiotrophoblast CS protein and maternal serum
CS levels. In contrast, placental villous
5-3ß-hydroxysteroid dehydrogenase, 11ß-hydroxysteroid
dehydrogenase-2, and P-450 aromatase protein levels
were unaltered by androstenedione or estradiol
treatment. Collectively, these results suggest that,
in elevated levels, estrogen suppressed CS formation
by villous syncytiotrophoblast during the first one
third of primate pregnancy. Therefore, estrogen has
very different and specific actions on steroid and
peptide hormone biosynthesis within the placental
trophoblast, which we propose are important in
regulating placental function and promoting
fetal-placental development in the primate. 

This work was supported by NIH Research Grant R01

Abbreviations: CS, Chorionic somatomammotropin;
11ß-HSD, 11ß-hydroxysteroid dehydrogenase; LDL,
low-density lipoprotein; P-450scc, P-450 cholesterol
side-chain cleavage; poly(A)+, polyadenylated (A)+;
PRL, prolactin.

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